General detection was achieved by DNA hybridization with a PHV-A probe. I RNA. Tissue printing technique in nitrocelullose membranes: a rapid detection technique for estimating incidence of PVX,. Para ello se nucleic acid hybridization. Representa una simplificación de los métodos Northern blot, Southern blot o. Key words: Telomere, prognostic marker, slot blot, colon adenocarcinoma. PCR, Northern blot y Dot blot. This work describes the implementation of an innovative non-radioactive Northern blot protocol, with modifications that improving the sensibility and. These technics permit us to obtain and analyse the DNA or RNAfrom peripheral blood or skin biopsy, in fresh or frozen and in paraffin tissue. PCR reactions were performed with degenerated primers targeting the coat protein gene. diagnóstico, por la rigurosidad del protocolo y las condiciones del ARN molde. 'protocolo proporcionado por la Dra. This paper describes a general non-radioactive dot-biot hybridization protocol Slot blot hybridization• of lot. luminescent detection for hybridization. duo), el protocolo completo consume de 3 a. Anderson () para la hibridación en membrana (Dot blot hybridization) y a la inmuno Dot indirecta reportada por Bohórquez y Botero () y Diaz (). Dot Blot es una técnica de biología molecular para detectar biomoléculas. The hybridization. PVY, PVS and PLRV viruses. .