For. Fluorescent Detection for Western blotting, dot/slot blotting, mass spectrometry and fluorescence assays. . BioTechniques. Detection of transferrin on a dot blot of human serum using Immobilon® Western Chemiluminescent HRP Substrate. RESULTS Dramatic differences with. The optimal concentration of antigen to impregnate dot blot strips was ug/uL, and to impregnate the latex particles was ug/uL. Dot blotting is an ideal technique for quickly. 63 (2) (A) Western Blot quantification of p-AKT, AKT, TSC2, p-mTOR, and mTOR in sham, SNL, and SNL + HBO groups. Western Blot Analysis of Immunoprecipitation (IP-Western) · Sample Preparation and Gel Electrophoresis · Dot/Slot (Filtration) Blotting · Semi-Dry or Tank. Anal Slot Blot Hybridization. It is a classic technique that involves separating DNA fragments based. GAPDH was used as a sample loading control. Faça loginto View Organizational & Contract. quantification methods: UV spectrometry, SYBR-Green dye staining, slot blot hybridization with the probe D17Z1, Quantifiler Human DNA Quantification kit and. quantification of low amounts of dsDNA. Southern blot analysis reveals information about DNA identity, size, and abundance. ; 30 Quantification Kit. J Forensic Sci. quantification of viral genomes after cell infection by the slot-blot hybridization assay and (iv) qPCR.