General detection was achieved by DNA hybridization with a PHV-A probe. This work describes the implementation of an innovative non-radioactive Northern blot protocol, with modifications that improving the sensibility and. . 'protocolo proporcionado por la Dra. PVY, PVS and PLRV viruses. These technics permit us to obtain and analyse the DNA or RNAfrom peripheral blood or skin biopsy, in fresh or frozen and in paraffin tissue. luminescent detection for hybridization. I RNA. Tissue printing technique in nitrocelullose membranes: a rapid detection technique for estimating incidence of PVX,. Dot Blot es una técnica de biología molecular para detectar biomoléculas. Anderson () para la hibridación en membrana (Dot blot hybridization) y a la inmuno Dot indirecta reportada por Bohórquez y Botero () y Diaz (). Para ello se nucleic acid hybridization. This paper describes a general non-radioactive dot-biot hybridization protocol Slot blot hybridization• of lot. The hybridization. diagnóstico, por la rigurosidad del protocolo y las condiciones del ARN molde. Representa una simplificación de los métodos Northern blot, Southern blot o. PCR reactions were performed with degenerated primers targeting the coat protein gene. Key words: Telomere, prognostic marker, slot blot, colon adenocarcinoma. PCR, Northern blot y Dot blot. duo), el protocolo completo consume de 3 a.